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il 13  (R&D Systems)


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    Structured Review

    R&D Systems il 13
    Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+13+protein/Recombinant+Human+IL-13+Protein/pmc13062519-322-19-24
    Average 96 stars, based on 222 article reviews
    il 13 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Effects of Indole-3-Lactic Acid, a Metabolite of Tryptophan, on IL-4 and IL-13-Induced Human Skin-Equivalent Atopic Dermatitis Models
    Article Snippet: .. The recombinant human IL-4 Protein and recombinant human IL-13 Protein (R&D system) treatment (15 ng/mL for both) was performed at the air exposure stage. ..

    Article Title: Targeting IL13Rα2 in melanoma with a bispecific T-cell engager: expression profiling and preclinical evaluation
    Article Snippet: Then Alexa Fluor 647 AffiniPure goat anti-human IgG, F(ab’)2 fragment specific (Jackson ImmunoResearch, 109-605-006) was used as secondary antibody. .. In the indicated experiment, recombinant human IL-13 protein (R&D systems, 213-ILB) was added before binding with PE anti-human IL13Rα2 antibody or bispecific antibodies. ..

    Article Title: An Interleukin-4 and Interleukin-13 Induced Atopic Dermatitis Human Skin Equivalent Model by a Skin-On-A-Chip
    Article Snippet: .. Recombinant human IL-4 protein and recombinant human IL-13 protein (R&D system, Minneapolis) treatment was performed at the air exposure stage. ..

    Article Title: IL-13 induces periostin and eotaxin expression in human primary alveolar epithelial cells: Comparison with paired airway epithelial cells
    Article Snippet: .. Recombinant human IL-13 protein from the R&D Systems, Minneapolis, MN was reconstituted in 0.1% bovine serum albumin (BSA) and stored at -80°C. .. Bronchial epithelial cell growth medium (BEGM) with antibiotics was purchased from Lonza, Walkersville, MD, USA.

    Article Title: Targeting IL13Rα2 in melanoma with a bispecific T-cell engager: expression profiling and preclinical evaluation.
    Article Snippet: Then Alexa Fluor 647 AffiniPure goat anti- human IgG, F(ab’)2 fragment specific (Jackson ImmunoResearch, 109- 605- 006) was used as secondary antibody. .. In the indicated experiment, recombinant human IL- 13 protein (R&D systems, 213- ILB) was added before binding with PE anti- human IL13Rα2 antibody or bispecific antibodies. ..

    Article Title: Mapping the druggable targets displayed by human colonic enteroendocrine cells
    Article Snippet: The organoids were then incubated for 2 hrs at 37 °C in 100 μL of saline secretion buffer containing 138 mM NaCl, 4.5 mM KCl, 4.2 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , 2.6 mM CaCl 2 , 1.2 mM MgCl 2 , 10 mM HEPES (pH 7.4), with freshly added 0.1% fatty acid free BSA (A6003, Sigma-Aldrich) and 50 μM DPP IV Inhibitor (DPP4, Sigma-Aldrich). .. Four groups were set up: a negative control, treatment of 10 μM Forskolin (FSK, F6886, Scientific Laboratory Supplies) and 10 μM IBMX (I5879, Merck), and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). ..

    Article Title: Mapping the druggable targets displayed by human colonic enteroendocrine cells
    Article Snippet: The organoids were then incubated for 2 hrs at 37°C in 100 μL of saline secretion buffer containing 138 mM NaCl, 4.5 mM KCl, 4.2 mM NaHCO3, 1.2 mM NaH2PO4, 2.6 mM CaCl2, 1.2 mM MgCl2, 10 mM HEPES (pH 7.4), with freshly added 0.1% fatty acid free BSA (A6003, Sigma-Aldrich) and 50 μM DPP IV Inhibitor (DPP4, Sigma-Aldrich). .. Four groups were set up: a negative control, treatment of 10 μM Forskolin (FSK, F6886, Scienti c Laboratory Supplies) and 10 μM IBMX (I5879, Merck), and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). ..

    Binding Assay:

    Article Title: Targeting IL13Rα2 in melanoma with a bispecific T-cell engager: expression profiling and preclinical evaluation
    Article Snippet: Then Alexa Fluor 647 AffiniPure goat anti-human IgG, F(ab’)2 fragment specific (Jackson ImmunoResearch, 109-605-006) was used as secondary antibody. .. In the indicated experiment, recombinant human IL-13 protein (R&D systems, 213-ILB) was added before binding with PE anti-human IL13Rα2 antibody or bispecific antibodies. ..

    Article Title: Targeting IL13Rα2 in melanoma with a bispecific T-cell engager: expression profiling and preclinical evaluation.
    Article Snippet: Then Alexa Fluor 647 AffiniPure goat anti- human IgG, F(ab’)2 fragment specific (Jackson ImmunoResearch, 109- 605- 006) was used as secondary antibody. .. In the indicated experiment, recombinant human IL- 13 protein (R&D systems, 213- ILB) was added before binding with PE anti- human IL13Rα2 antibody or bispecific antibodies. ..

    Negative Control:

    Article Title: Mapping the druggable targets displayed by human colonic enteroendocrine cells
    Article Snippet: The organoids were then incubated for 2 hrs at 37 °C in 100 μL of saline secretion buffer containing 138 mM NaCl, 4.5 mM KCl, 4.2 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , 2.6 mM CaCl 2 , 1.2 mM MgCl 2 , 10 mM HEPES (pH 7.4), with freshly added 0.1% fatty acid free BSA (A6003, Sigma-Aldrich) and 50 μM DPP IV Inhibitor (DPP4, Sigma-Aldrich). .. Four groups were set up: a negative control, treatment of 10 μM Forskolin (FSK, F6886, Scientific Laboratory Supplies) and 10 μM IBMX (I5879, Merck), and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). ..

    Article Title: Mapping the druggable targets displayed by human colonic enteroendocrine cells
    Article Snippet: The organoids were then incubated for 2 hrs at 37°C in 100 μL of saline secretion buffer containing 138 mM NaCl, 4.5 mM KCl, 4.2 mM NaHCO3, 1.2 mM NaH2PO4, 2.6 mM CaCl2, 1.2 mM MgCl2, 10 mM HEPES (pH 7.4), with freshly added 0.1% fatty acid free BSA (A6003, Sigma-Aldrich) and 50 μM DPP IV Inhibitor (DPP4, Sigma-Aldrich). .. Four groups were set up: a negative control, treatment of 10 μM Forskolin (FSK, F6886, Scienti c Laboratory Supplies) and 10 μM IBMX (I5879, Merck), and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). ..



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    R&D Systems recombinant human il 13
    a A549 cells were treated with rhSP-D (10 mg/ml) for 2 h, followed by incubation with recombinant human IL-4 (10 ng/ml) <t>or</t> <t>IL-13</t> (10 ng/ml) for 15 min before harvest. Protein expression and phosphorylation were assessed by western blotting. b Interleukin 4 receptor α (IL-4R-α) and BSA were coated (1 mg/ml) in 96 ELISA plates and different concentrations of rhSP-D were added. After 2 h, the relative binding was evaluated by colourimetic ELISA assay (optical density (OD)). c Representative surface plasmon resonance fits of binding of rhSP-D in the concentration range 1.5–200 nM to immobilized IL-4R-α. Experimental data, expressed as resonance units (RU), are shown as solid lines and fitted curves as dotted lines. The following parameters were determined; k a = 7.1 ± 1.9 × 10 3 /Ms, k d = 1.7 ± 0.4 × 10 −4 /s, K D = 25 ± 5 nM. Average chi 2 / R max of the fits was 0.011. d Dual injection on the IL-4R sensor chip. First injection with 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (purple), 100 mM mannose (blue), or 100 mM N-acetyl-D-glucosamine (GluNac) (red). e Association-phase of the injection of 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (green), 1–100 mM mannose (blue) or 1–100 mM N-acetyl-D-glucosamine (GluNac) (red).
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    Image Search Results


    a A549 cells were treated with rhSP-D (10 mg/ml) for 2 h, followed by incubation with recombinant human IL-4 (10 ng/ml) or IL-13 (10 ng/ml) for 15 min before harvest. Protein expression and phosphorylation were assessed by western blotting. b Interleukin 4 receptor α (IL-4R-α) and BSA were coated (1 mg/ml) in 96 ELISA plates and different concentrations of rhSP-D were added. After 2 h, the relative binding was evaluated by colourimetic ELISA assay (optical density (OD)). c Representative surface plasmon resonance fits of binding of rhSP-D in the concentration range 1.5–200 nM to immobilized IL-4R-α. Experimental data, expressed as resonance units (RU), are shown as solid lines and fitted curves as dotted lines. The following parameters were determined; k a = 7.1 ± 1.9 × 10 3 /Ms, k d = 1.7 ± 0.4 × 10 −4 /s, K D = 25 ± 5 nM. Average chi 2 / R max of the fits was 0.011. d Dual injection on the IL-4R sensor chip. First injection with 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (purple), 100 mM mannose (blue), or 100 mM N-acetyl-D-glucosamine (GluNac) (red). e Association-phase of the injection of 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (green), 1–100 mM mannose (blue) or 1–100 mM N-acetyl-D-glucosamine (GluNac) (red).

    Journal: NPJ Precision Oncology

    Article Title: Pulmonary surfactant protein D reduces lung cancer progression associated with decreased IL-4/STAT6 signaling

    doi: 10.1038/s41698-025-01163-6

    Figure Lengend Snippet: a A549 cells were treated with rhSP-D (10 mg/ml) for 2 h, followed by incubation with recombinant human IL-4 (10 ng/ml) or IL-13 (10 ng/ml) for 15 min before harvest. Protein expression and phosphorylation were assessed by western blotting. b Interleukin 4 receptor α (IL-4R-α) and BSA were coated (1 mg/ml) in 96 ELISA plates and different concentrations of rhSP-D were added. After 2 h, the relative binding was evaluated by colourimetic ELISA assay (optical density (OD)). c Representative surface plasmon resonance fits of binding of rhSP-D in the concentration range 1.5–200 nM to immobilized IL-4R-α. Experimental data, expressed as resonance units (RU), are shown as solid lines and fitted curves as dotted lines. The following parameters were determined; k a = 7.1 ± 1.9 × 10 3 /Ms, k d = 1.7 ± 0.4 × 10 −4 /s, K D = 25 ± 5 nM. Average chi 2 / R max of the fits was 0.011. d Dual injection on the IL-4R sensor chip. First injection with 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (purple), 100 mM mannose (blue), or 100 mM N-acetyl-D-glucosamine (GluNac) (red). e Association-phase of the injection of 100 nM rhSP-D followed by addition of either 5 mM CaCl 2 (black), 10 mM EDTA (green), 1–100 mM mannose (blue) or 1–100 mM N-acetyl-D-glucosamine (GluNac) (red).

    Article Snippet: A549 cells were treated with rhSP-D (50 μg/ml) for 2 h. Following, the cells were stimulated by recombinant human IL-4 (R&D systems, 6507-IL) or recombinant human IL-13 (R&D systems, 213-ILB) for 15 min.

    Techniques: Incubation, Recombinant, Expressing, Phospho-proteomics, Western Blot, Enzyme-linked Immunosorbent Assay, Binding Assay, SPR Assay, Concentration Assay, Injection